| ORIGINAL ARTICLE |
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| Year : 2014 | Volume
: 2
| Issue : 2 | Page : 33-38 |
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A comparative study of the preventive effect of chlorhexidine o. 12% and nano zinc oxide particles on the distraction of collagen scaffolding of the hybrid layer by two immunohistochemistry and microleakage tests
Homayoon Alaghemand1, Behnaz Esmaeili1, Pouralibaba Firouz2, Mohammad Hossein Soltani1, Marzyeh Rouhaninasab1, Ali Dehghani nazhvani3, Ali Bijani4
1 Department of Oral Medicine, Dental materials Research Center, Babol University of Medical Science, Babol, Iran
2 Department of Oral Medicine, Tabriz University of Medical Science, Tabriz, Iran
3 Department of Esthetic and Restorative Dentistry, Babol University of Medical Science, Babol, Iran
4 Department of Oral Pathology, Shiraz University of Medical Science, Shiraz, Social Determinant of Health Research Center, Babol University of Medical Science, Babol, Iran
Correspondence Address:
Mohammad Hossein Soltani
Department of Esthetic and Restorative Dentistry, Babol University of Medical Science, Babol
Iran
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/2348-1471.143327
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Introduction: Hybrid layer degradation is mainly attributed to matrix metalo proteinases (MMP) activity. In this study, we compare inhibitory effect of nano zinc oxide with chlorhexidine (0.12%) on distraction of collagen scaffolding of the hybrid layer by immunohistochemistry (IHC) and microleakage tests. Materials and Methods: A total of 60 extracted molars and premolar teeth with no caries and visible anatomic defects were selected. Class 5 cavities with dimensions of 2 × 2 × 2 mm was performed for each tooth on the buccal surface 1 mm above cemento enamel junction. Results: In microleakage test, there was no significant difference in the result for groups pretreated with nano zinc oxide mixed with single bond (C), pretreated with chlorhexidine (0.12%) applied before bonding (B) and not pretreated with these agents (A) whether in 24 h or 1-week. in IHC test, there was a significant difference (P < 0.05) between all groups except in group B and group C. Conclusion: (1) The inhibitory effect of chlorhexidine and nano zinc oxide on MMPs activity is different. (2) Microleakage amount is different in composite resin restoration used in a cavity pretreated with nano zinc oxide contained bonding or chlorhexidine 24 h and 1-week later. |
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